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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Zea mays ATP synthase subunit a, chloroplastic (atpI), partial CSB-YP323504ZAX1
CSB-EP323504ZAX1
CSB-BP323504ZAX1
CSB-MP323504ZAX1
CSB-EP323504ZAX1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Uncharacterized protein 16 (VACWR016) CSB-YP323505VAI
CSB-EP323505VAI
CSB-BP323505VAI
CSB-MP323505VAI
CSB-EP323505VAI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Protein C8L (VACWR020) CSB-YP323506VAI
CSB-EP323506VAI
CSB-BP323506VAI
CSB-MP323506VAI
CSB-EP323506VAI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Kelch repeat protein C2 (VACWR026), partial CSB-YP323507VAI
CSB-EP323507VAI
CSB-BP323507VAI
CSB-MP323507VAI
CSB-EP323507VAI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Replication protein E1 (E1), partial CSB-YP323508HND
CSB-EP323508HND
CSB-BP323508HND
CSB-MP323508HND
CSB-EP323508HND-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Minor capsid protein L2 (L2) CSB-YP323509HND
CSB-EP323509HND
CSB-BP323509HND
CSB-MP323509HND
CSB-EP323509HND-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hepatitis B virus genotype B2 Large envelope protein (S), partial CSB-YP323510HDW1
CSB-EP323510HDW1
CSB-BP323510HDW1
CSB-MP323510HDW1
CSB-EP323510HDW1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dichelobacter nodosus Probable minor fimbrial protein (fimZ) CSB-YP323512DDR
CSB-EP323512DDR
CSB-BP323512DDR
CSB-MP323512DDR
CSB-EP323512DDR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Phosphate system positive regulatory protein PHO81 (PHO81), partial CSB-YP323513SVG
CSB-EP323513SVG
CSB-BP323513SVG
CSB-MP323513SVG
CSB-EP323513SVG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dermonecrotic toxin (toxA), partial CSB-YP323514ESF
CSB-EP323514ESF
CSB-BP323514ESF
CSB-MP323514ESF
CSB-EP323514ESF-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Turnip crinkle virus RNA-directed RNA polymerase (ORF1), partial CSB-YP323515TJL
CSB-EP323515TJL
CSB-BP323515TJL
CSB-MP323515TJL
CSB-EP323515TJL-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Outer capsid glycoprotein VP7 CSB-YP323516RON
CSB-EP323516RON
CSB-BP323516RON
CSB-MP323516RON
CSB-EP323516RON-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equine herpesvirus 1 Trans-acting transcriptional protein ICP4 (IE), partial CSB-YP323517EFM
CSB-EP323517EFM
CSB-BP323517EFM
CSB-MP323517EFM
CSB-EP323517EFM-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Replication protein CSB-YP323518NFZ
CSB-EP323518NFZ
CSB-BP323518NFZ
CSB-MP323518NFZ
CSB-EP323518NFZ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium falciparum Sexual stage-specific protein, partial CSB-YP323519PMB1
CSB-EP323519PMB1
CSB-BP323519PMB1
CSB-MP323519PMB1
CSB-EP323519PMB1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Metallothionein-2 (mtl-2) CSB-YP323520CXY
CSB-EP323520CXY
CSB-BP323520CXY
CSB-MP323520CXY
CSB-EP323520CXY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Potato leafroll virus Protein P1 (ORF1), partial CSB-YP323521PQW1
CSB-EP323521PQW1
CSB-BP323521PQW1
CSB-MP323521PQW1
CSB-EP323521PQW1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Potato virus M Movement protein TGB2 (ORF3), partial CSB-YP323522PRA1
CSB-EP323522PRA1
CSB-BP323522PRA1
CSB-MP323522PRA1
CSB-EP323522PRA1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Tropomyosin-1 (TPM1) CSB-YP323523SVG
CSB-EP323523SVG
CSB-BP323523SVG
CSB-MP323523SVG
CSB-EP323523SVG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Protein ChrB (chrB1) CSB-YP323524RJG
CSB-EP323524RJG
CSB-BP323524RJG
CSB-MP323524RJG
CSB-EP323524RJG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過(guò)多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.jhhuazhong.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn)，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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