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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mouse Cell division control protein 42 homolog (Cdc42) CSB-EP005008MO 1mg/100ug/20ug
Recombinant Pisum sativum Gibberellin 3-beta-dioxygenase 1 (LE) (A229T) CSB-EP7159HZY(M) 1mg/100ug/20ug
Recombinant Pisum sativum Gibberellin 3-beta-dioxygenase 1 (LE) CSB-EP7159HZY 1mg/100ug/20ug
Recombinant Human Transcription factor COE3 (EBF3) CSB-EP880977HUb0 1mg/100ug/20ug
Recombinant Aspergillus oryzae Probable?pectate?lyase?E (plyE) CSB-EP652707DPA 1mg/100ug/20ug
Recombinant?Bovine?Galectin?(LGALS3) CSB-EP7276BO 1mg/100ug/20ug
Recombinant Human Filamin-B (FLNB), partial CSB-EP008725HU1 1mg/100ug/20ug
Recombinant Human Filamin-B (FLNB) (T2177A,V2354A,R2374A), partial CSB-EP008725HU1(M) 1mg/100ug/20ug
Recombinant Staphylococcus epidermidis Glutamyl endopeptidase (gseA) CSB-EP314220SLD 1mg/100ug/20ug
Recombinant Pig Pepsin A (PGA) CSB-EP360581PI 1mg/100ug/20ug
Recombinant Simian retrovirus SRV-2 Gag-Pro polyprotein (pro), partial CSB-EP344347SHN 1mg/100ug/20ug
Recombinant Thermoplasma acidophilum Tricorn protease (tri), partial CSB-EP309466TND 1mg/100ug/20ug
Recombinant Mouse Cytosolic carboxypeptidase 6 (Agbl4) CSB-EP600702MO 1mg/100ug/20ug
Recombinant Pig Natriuretic peptides A (NPPA), partial, Biotinylated CSB-EP016020PI-B 1mg/100ug/20ug
Recombinant Human HLA class I histocompatibility antigen, C alpha chain (HLA-C), partial CSB-EP326978HU 1mg/100ug/20ug
Recombinant Escherichia coli Transposase for transposon Tn5 (tnpA) (E54K,L372P) CSB-EP669378ENL(M)h9 1mg/100ug/20ug
Recombinant Human Zinc finger protein 746 (ZNF746) CSB-EP027008HU 1mg/100ug/20ug
Recombinant Danio rerio E3 ubiquitin-protein ligase TRIM33 (trim33), partial CSB-EP724971DIL 1mg/100ug/20ug
Recombinant Human Interleukin-20 receptor subunit alpha (IL20RA), partial CSB-EP887035HU 1mg/100ug/20ug
Recombinant Bartonella henselae Type IV secretion system protein virB4 (virB4), partial CSB-EP870829BSG 1mg/100ug/20ug

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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