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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae Chitin synthase 3 (CHS3), partial CSB-YP341069SVG
CSB-EP341069SVG
CSB-BP341069SVG
CSB-MP341069SVG
CSB-EP341069SVG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Toxin coregulated pilus biosynthesis protein S (tcpS) CSB-YP341071VEX
CSB-EP341071VEX
CSB-BP341071VEX
CSB-MP341071VEX
CSB-EP341071VEX-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Minichromosome maintenance protein 5 (MCM5), partial CSB-YP341072SVG
CSB-EP341072SVG
CSB-BP341072SVG
CSB-MP341072SVG
CSB-EP341072SVG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Tubulin beta-2 chain (TUBB2) CSB-YP341073EWE
CSB-EP341073EWE
CSB-BP341073EWE
CSB-MP341073EWE
CSB-EP341073EWE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Tubulin alpha-6 chain (TUBA6) CSB-YP341074DOA
CSB-EP341074DOA
CSB-BP341074DOA
CSB-MP341074DOA
CSB-EP341074DOA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Protein brittle-1, chloroplastic/amyloplastic (BT1), partial CSB-YP341075ZAX1
CSB-EP341075ZAX1
CSB-BP341075ZAX1
CSB-MP341075ZAX1
CSB-EP341075ZAX1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis General transcription factor IIE subunit 2 (gtf2e2) CSB-YP341076XBE
CSB-EP341076XBE
CSB-BP341076XBE
CSB-MP341076XBE
CSB-EP341076XBE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum Elongation factor 1-beta CSB-YP341077TQN
CSB-EP341077TQN
CSB-BP341077TQN
CSB-MP341077TQN
CSB-EP341077TQN-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aedes aegypti Homeobox protein abdominal-A homolog (abd-A) CSB-YP341078AXQ
CSB-EP341078AXQ
CSB-BP341078AXQ
CSB-MP341078AXQ
CSB-EP341078AXQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Modification methylase MthTI (mthTIM) CSB-YP341079MEV
CSB-EP341079MEV
CSB-BP341079MEV
CSB-MP341079MEV
CSB-EP341079MEV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein ORF4 CSB-YP341080MEV
CSB-EP341080MEV
CSB-BP341080MEV
CSB-MP341080MEV
CSB-EP341080MEV-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Putative hexose transport activator protein (AHT1) CSB-YP341082SVG
CSB-EP341082SVG
CSB-BP341082SVG
CSB-MP341082SVG
CSB-EP341082SVG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ferredoxin CSB-YP341083TMQ
CSB-EP341083TMQ
CSB-BP341083TMQ
CSB-MP341083TMQ
CSB-EP341083TMQ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Serine protease inhibitor A3C (Serpina3c) CSB-YP341084MO
CSB-EP341084MO
CSB-BP341084MO
CSB-MP341084MO
CSB-EP341084MO-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hevea brasiliensis Ribulose bisphosphate carboxylase small chain, chloroplastic (RBCS) CSB-YP341085HWI
CSB-EP341085HWI
CSB-BP341085HWI
CSB-MP341085HWI
CSB-EP341085HWI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 17 kDa surface antigen (omp) CSB-YP341086RET
CSB-EP341086RET
CSB-BP341086RET
CSB-MP341086RET
CSB-EP341086RET-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase epsilon chain, sodium ion specific (atpC) CSB-YP341087PSI
CSB-EP341087PSI
CSB-BP341087PSI
CSB-MP341087PSI
CSB-EP341087PSI-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum 17 kDa lipoprotein (tpp17) CSB-YP341088TPR
CSB-EP341088TPR
CSB-BP341088TPR
CSB-MP341088TPR
CSB-EP341088TPR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mastigocladus laminosus Bilin biosynthesis protein PecE (pecE) CSB-YP341089MQJ
CSB-EP341089MQJ
CSB-BP341089MQJ
CSB-MP341089MQJ
CSB-EP341089MQJ-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Photobacterium profundum UPF0319 protein PBPRA1013 (PBPRA1013) CSB-YP341091PIG
CSB-EP341091PIG
CSB-BP341091PIG
CSB-MP341091PIG
CSB-EP341091PIG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中，除了表達系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達載體?

表達載體是一種DNA分子，它攜帶特定的基因進入宿主細胞，并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達，經(jīng)過多年的發(fā)展，表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示，本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條：如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.jhhuazhong.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達服務，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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