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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Francisella tularensis subsp. novicida Dihydroxy-acid dehydratase (ilvD), partial CSB-YP367815FDW
CSB-EP367815FDW
CSB-BP367815FDW
CSB-MP367815FDW
CSB-EP367815FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP367816FDW
CSB-EP367816FDW
CSB-BP367816FDW
CSB-MP367816FDW
CSB-EP367816FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP367817FDW
CSB-EP367817FDW
CSB-BP367817FDW
CSB-MP367817FDW
CSB-EP367817FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida 3-dehydroquinate synthase (aroB) CSB-YP367818FDW
CSB-EP367818FDW
CSB-BP367818FDW
CSB-MP367818FDW
CSB-EP367818FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida SsrA-binding protein (smpB) CSB-YP367819FDW
CSB-EP367819FDW
CSB-BP367819FDW
CSB-MP367819FDW
CSB-EP367819FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida 50S ribosomal protein L20 (rplT) CSB-YP367820FDW
CSB-EP367820FDW
CSB-BP367820FDW
CSB-MP367820FDW
CSB-EP367820FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida UPF0133 protein FTN_1196 (FTN_1196) CSB-YP367821FDW
CSB-EP367821FDW
CSB-BP367821FDW
CSB-MP367821FDW
CSB-EP367821FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida DNA repair protein recO (recO) CSB-YP367822FDW
CSB-EP367822FDW
CSB-BP367822FDW
CSB-MP367822FDW
CSB-EP367822FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Ribonuclease HII (rnhB) CSB-YP367823FDW
CSB-EP367823FDW
CSB-BP367823FDW
CSB-MP367823FDW
CSB-EP367823FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Phosphoglycerate kinase (pgk) CSB-YP367824FDW
CSB-EP367824FDW
CSB-BP367824FDW
CSB-MP367824FDW
CSB-EP367824FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida DNA ligase (ligA), partial CSB-YP367825FDW
CSB-EP367825FDW
CSB-BP367825FDW
CSB-MP367825FDW
CSB-EP367825FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Pantothenate synthetase (panC) CSB-YP367826FDW
CSB-EP367826FDW
CSB-BP367826FDW
CSB-MP367826FDW
CSB-EP367826FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida DNA-directed RNA polymerase subunit alpha 2 (rpoA2) CSB-YP367827FDW
CSB-EP367827FDW
CSB-BP367827FDW
CSB-MP367827FDW
CSB-EP367827FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Lipid-A-disaccharide synthase (lpxB) CSB-YP367828FDW
CSB-EP367828FDW
CSB-BP367828FDW
CSB-MP367828FDW
CSB-EP367828FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida N-acetylmuramic acid 6-phosphate etherase (murQ) CSB-YP367829FDW
CSB-EP367829FDW
CSB-BP367829FDW
CSB-MP367829FDW
CSB-EP367829FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida DNA mismatch repair protein MutS (mutS), partial CSB-YP367830FDW
CSB-EP367830FDW
CSB-BP367830FDW
CSB-MP367830FDW
CSB-EP367830FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP367831FDW
CSB-EP367831FDW
CSB-BP367831FDW
CSB-MP367831FDW
CSB-EP367831FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida UPF0246 protein FTN_1542 (FTN_1542) CSB-YP367832FDW
CSB-EP367832FDW
CSB-BP367832FDW
CSB-MP367832FDW
CSB-EP367832FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP367833FDW
CSB-EP367833FDW
CSB-BP367833FDW
CSB-MP367833FDW
CSB-EP367833FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. novicida 50S ribosomal protein L19 (rplS) CSB-YP367834FDW
CSB-EP367834FDW
CSB-BP367834FDW
CSB-MP367834FDW
CSB-EP367834FDW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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