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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycobacterium sp. Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP379080MOM
CSB-EP379080MOM
CSB-BP379080MOM
CSB-MP379080MOM
CSB-EP379080MOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. UPF0061 protein Mkms_5290 (Mkms_5290) CSB-YP379081MOM
CSB-EP379081MOM
CSB-BP379081MOM
CSB-MP379081MOM
CSB-EP379081MOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 30S ribosomal protein S18 (rpsR) CSB-YP379082MOM
CSB-EP379082MOM
CSB-BP379082MOM
CSB-MP379082MOM
CSB-EP379082MOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. Leucine--tRNA ligase (leuS), partial CSB-YP379083MOM
CSB-EP379083MOM
CSB-BP379083MOM
CSB-MP379083MOM
CSB-EP379083MOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium sp. 4-hydroxy-2-oxovalerate aldolase 2 (Mkms_5835) CSB-YP379084MOM
CSB-EP379084MOM
CSB-BP379084MOM
CSB-MP379084MOM
CSB-EP379084MOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis Putative phosphotransferase BARBAKC583_0008 (BARBAKC583_0008) CSB-YP379085BOR
CSB-EP379085BOR
CSB-BP379085BOR
CSB-MP379085BOR
CSB-EP379085BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis tRNA modification GTPase MnmE (mnmE) CSB-YP379086BOR
CSB-EP379086BOR
CSB-BP379086BOR
CSB-MP379086BOR
CSB-EP379086BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP379087BOR
CSB-EP379087BOR
CSB-BP379087BOR
CSB-MP379087BOR
CSB-EP379087BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP379088BOR
CSB-EP379088BOR
CSB-BP379088BOR
CSB-MP379088BOR
CSB-EP379088BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis 30S ribosomal protein S16 (rpsP) CSB-YP379089BOR
CSB-EP379089BOR
CSB-BP379089BOR
CSB-MP379089BOR
CSB-EP379089BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP379090BOR
CSB-EP379090BOR
CSB-BP379090BOR
CSB-MP379090BOR
CSB-EP379090BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis 50S ribosomal protein L28 (rpmB) CSB-YP379091BOR
CSB-EP379091BOR
CSB-BP379091BOR
CSB-MP379091BOR
CSB-EP379091BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis tRNA (guanine-N (1)-)-methyltransferase CSB-YP379092BOR
CSB-EP379092BOR
CSB-BP379092BOR
CSB-MP379092BOR
CSB-EP379092BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis ATP synthase gamma chain (atpG) CSB-YP379093BOR
CSB-EP379093BOR
CSB-BP379093BOR
CSB-MP379093BOR
CSB-EP379093BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP379094BOR
CSB-EP379094BOR
CSB-BP379094BOR
CSB-MP379094BOR
CSB-EP379094BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis UPF0335 protein BARBAKC583_0130 (BARBAKC583_0130) CSB-YP379095BOR
CSB-EP379095BOR
CSB-BP379095BOR
CSB-MP379095BOR
CSB-EP379095BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP379096BOR
CSB-EP379096BOR
CSB-BP379096BOR
CSB-MP379096BOR
CSB-EP379096BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP379097BOR
CSB-EP379097BOR
CSB-BP379097BOR
CSB-MP379097BOR
CSB-EP379097BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis 50S ribosomal protein L31 (rpmE) CSB-YP379098BOR
CSB-EP379098BOR
CSB-BP379098BOR
CSB-MP379098BOR
CSB-EP379098BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bartonella bacilliformis ATP-dependent zinc metalloprotease FtsH (ftsH), partial CSB-YP379099BOR
CSB-EP379099BOR
CSB-BP379099BOR
CSB-MP379099BOR
CSB-EP379099BOR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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