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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Actinobacillus succinogenes UPF0042 nucleotide-binding protein Asuc_0930 (Asuc_0930) CSB-YP407930AUG
CSB-EP407930AUG
CSB-BP407930AUG
CSB-MP407930AUG
CSB-EP407930AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Putative ribosome biogenesis GTPase RsgA (rsgA) CSB-YP407931AUG
CSB-EP407931AUG
CSB-BP407931AUG
CSB-MP407931AUG
CSB-EP407931AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Serine--tRNA ligase (serS) CSB-YP407932AUG
CSB-EP407932AUG
CSB-BP407932AUG
CSB-MP407932AUG
CSB-EP407932AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes tRNA (Ile)-lysidine synthase CSB-YP407933AUG
CSB-EP407933AUG
CSB-BP407933AUG
CSB-MP407933AUG
CSB-EP407933AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Citrate lyase acyl carrier protein (citD) CSB-YP407934AUG
CSB-EP407934AUG
CSB-BP407934AUG
CSB-MP407934AUG
CSB-EP407934AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Threonine--tRNA ligase (thrS) CSB-YP407935AUG
CSB-EP407935AUG
CSB-BP407935AUG
CSB-MP407935AUG
CSB-EP407935AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Transcriptional repressor NrdR (nrdR) CSB-YP407936AUG
CSB-EP407936AUG
CSB-BP407936AUG
CSB-MP407936AUG
CSB-EP407936AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP407937AUG
CSB-EP407937AUG
CSB-BP407937AUG
CSB-MP407937AUG
CSB-EP407937AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP407938AUG
CSB-EP407938AUG
CSB-BP407938AUG
CSB-MP407938AUG
CSB-EP407938AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Tryptophan synthase beta chain (trpB) CSB-YP407939AUG
CSB-EP407939AUG
CSB-BP407939AUG
CSB-MP407939AUG
CSB-EP407939AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Hydroxyethylthiazole kinase (thiM) CSB-YP407940AUG
CSB-EP407940AUG
CSB-BP407940AUG
CSB-MP407940AUG
CSB-EP407940AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Deoxyguanosinetriphosphate triphosphohydrolase-like protein (Asuc_1615) CSB-YP407941AUG
CSB-EP407941AUG
CSB-BP407941AUG
CSB-MP407941AUG
CSB-EP407941AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP407942AUG
CSB-EP407942AUG
CSB-BP407942AUG
CSB-MP407942AUG
CSB-EP407942AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Glutamate-1-semialdehyde 2,1-aminomutase (hemL) CSB-YP407943AUG
CSB-EP407943AUG
CSB-BP407943AUG
CSB-MP407943AUG
CSB-EP407943AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Protein TolB (tolB) CSB-YP407944AUG
CSB-EP407944AUG
CSB-BP407944AUG
CSB-MP407944AUG
CSB-EP407944AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Sulfoxide reductase catalytic subunit YedY (yedY) CSB-YP407945AUG
CSB-EP407945AUG
CSB-BP407945AUG
CSB-MP407945AUG
CSB-EP407945AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Lipoprotein signal peptidase (lspA), partial CSB-YP407946AUG1
CSB-EP407946AUG1
CSB-BP407946AUG1
CSB-MP407946AUG1
CSB-EP407946AUG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP407947AUG
CSB-EP407947AUG
CSB-BP407947AUG
CSB-MP407947AUG
CSB-EP407947AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP407948AUG
CSB-EP407948AUG
CSB-BP407948AUG
CSB-MP407948AUG
CSB-EP407948AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes NADH pyrophosphatase (nudC) CSB-YP407949AUG
CSB-EP407949AUG
CSB-BP407949AUG
CSB-MP407949AUG
CSB-EP407949AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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