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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Methanococcus maripaludis Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP412863MNR
CSB-EP412863MNR
CSB-BP412863MNR
CSB-MP412863MNR
CSB-EP412863MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Dihydroxy-acid dehydratase (ilvD), partial CSB-YP412864MNR
CSB-EP412864MNR
CSB-BP412864MNR
CSB-MP412864MNR
CSB-EP412864MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis F420-dependent methylenetetrahydromethanopterin dehydrogenase (mtd) CSB-YP412865MNR
CSB-EP412865MNR
CSB-BP412865MNR
CSB-MP412865MNR
CSB-EP412865MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Threonine--tRNA ligase (thrS), partial CSB-YP412866MNR
CSB-EP412866MNR
CSB-BP412866MNR
CSB-MP412866MNR
CSB-EP412866MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 30S ribosomal protein S27ae (rps27Ae) CSB-YP412867MNR
CSB-EP412867MNR
CSB-BP412867MNR
CSB-MP412867MNR
CSB-EP412867MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis Dihydrodipicolinate synthase (dapA) CSB-YP412868MNR
CSB-EP412868MNR
CSB-BP412868MNR
CSB-MP412868MNR
CSB-EP412868MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 7-cyano-7-deazaguanine tRNA-ribosyltransferase (tgtA), partial CSB-YP412869MNR
CSB-EP412869MNR
CSB-BP412869MNR
CSB-MP412869MNR
CSB-EP412869MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis 30S ribosomal protein S2P (rps2p) CSB-YP412870MNR
CSB-EP412870MNR
CSB-BP412870MNR
CSB-MP412870MNR
CSB-EP412870MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanococcus maripaludis UDP-N-acetyl-D-mannosamine dehydrogenase (wecC) CSB-YP412871MNR
CSB-EP412871MNR
CSB-BP412871MNR
CSB-MP412871MNR
CSB-EP412871MNR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes 50S ribosomal protein L33 (rpmG) CSB-YP412872AUG
CSB-EP412872AUG
CSB-BP412872AUG
CSB-MP412872AUG
CSB-EP412872AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes High frequency lysogenization protein HflD homolog (hflD) CSB-YP412873AUG
CSB-EP412873AUG
CSB-BP412873AUG
CSB-MP412873AUG
CSB-EP412873AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Uronate isomerase (uxaC) CSB-YP412874AUG
CSB-EP412874AUG
CSB-BP412874AUG
CSB-MP412874AUG
CSB-EP412874AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes 23S rRNA (uracil (747)-C (5))-methyltransferase RlmC CSB-YP412875AUG
CSB-EP412875AUG
CSB-BP412875AUG
CSB-MP412875AUG
CSB-EP412875AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes 33 kDa chaperonin (hslO) CSB-YP412876AUG
CSB-EP412876AUG
CSB-BP412876AUG
CSB-MP412876AUG
CSB-EP412876AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Shikimate kinase (aroK) CSB-YP412877AUG
CSB-EP412877AUG
CSB-BP412877AUG
CSB-MP412877AUG
CSB-EP412877AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes ATP synthase gamma chain (atpG) CSB-YP412878AUG
CSB-EP412878AUG
CSB-BP412878AUG
CSB-MP412878AUG
CSB-EP412878AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes D-tyrosyl-tRNA (Tyr) deacylase CSB-YP412879AUG
CSB-EP412879AUG
CSB-BP412879AUG
CSB-MP412879AUG
CSB-EP412879AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP412880AUG
CSB-EP412880AUG
CSB-BP412880AUG
CSB-MP412880AUG
CSB-EP412880AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Met repressor (metJ) CSB-YP412881AUG
CSB-EP412881AUG
CSB-BP412881AUG
CSB-MP412881AUG
CSB-EP412881AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Orotate phosphoribosyltransferase (pyrE) CSB-YP412882AUG
CSB-EP412882AUG
CSB-BP412882AUG
CSB-MP412882AUG
CSB-EP412882AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.jhhuazhong.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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