(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Western blot
All lanes: ADH1B antibody at 2µg/ml + HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 40, 36 kDa
Observed band size: 40 kDa

Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA001354HA01HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA001354HA01HU at dilution of 1:100

Immunofluorescence staining of HepG2 cells with CSB-PA001354HA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).