(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Western Blot
Positive WB detected in: NIH/3T3 whole cell lysate(30μg)
All lanes:Aquaporin 1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 29 kDa
Observed band size: 40 kDa
Exposure time：2min

IHC image of CSB-RA975044A0HU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Western blot
All lanes: AQP1 antibody at 16µg/ml
Lane 1: Rat heart tissue
Lane 2: Mouse skeletal muscle tissue
Lane 3: A549 whole cell lysate
Lane 4: 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 29, 20, 24, 17 kDa
Observed band size: 29 kDa

Immunofluorescent analysis of HepG2 cells using CSB-PA16085A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunoprecipitating AQP1 in Mouse skeletal muscle tissue
Lane 1: Rabbit control IgG instead of CSB-PA16085A0Rb in Mouse skeletal muscle tissue. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA16085A0Rb (8µg) + Mouse skeletal muscle tissue (500µg)
Lane 3: Mouse skeletal muscle tissue (10µg)