(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized human GPC3 at 5 μg/ml can bind Anti-GPC3 recombinant antibody, the EC₅₀ is 35.19-52.30 ng/ml.

The Binding Activity of Human GPC3 with Anti-GPC3 recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human GPC3 (CSB-MP009705HU(M)) at 2 μg/mL can bind Anti- GPC3 recombinant Antibody, the EC₅₀ is 2.783-3.653 ng/mL.

Western Blot
Positive WB detected in: GPC3 antibody at 1:1000
Lane 1: Jurkat whole cell lysate
Lane 2: Mouse liver tissue
Secondary
Goat polyclonal to Mouse IgG at 1/20000 dilution
Predicted band size: 65-70KDa
Observed band size: 65-70 KDa
Exposure time: 5min

Overlay Peak curve showing Jurkat cells stained with CSB-MA009705A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.