(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human PAX8 at 2 μg/ml can bind Anti-PAX8 recombinant antibody (CSB-RA017494MA1HU). The EC₅₀ is 0.6290-0.7413 ng/mL.

The Binding Activity of Human PAX8 with Anti-PAX8 Recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human PAX8 (CSB-MP017494HU2) at 2 μg/mL can bind Anti-PAX8 recombinant antibody. The EC₅₀ is 0.6325-0.7353 ng/mL.

Overlay Peak curve showing 786-O cells stained with CSB-RA017494MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG1 (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA919764(PAX8 Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: ×200)

Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane 1-2: MCF7 cells, mouse brain tissue, Primary antibody: CSB-PA919764(PAX8 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes