Flow cytometric analysis of SMAD1 expression in HAP-1 cells using SMAD1 antibody. Green, isotype control; red, SMAD1.

Immunocytochemical staining of HAP-1 cells with SMAD1 antibody. Nuclei were stained blue with DAPI; SMAD1 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using SMAD1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMAD1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Immunohistochemistry of paraffin-embedded human skin tissue using CSB-PA618998LA01HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA618998LA01HU at dilution of 1:100

Western Blot
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, Raji whole cell lysate, HepG2 whole cell lysate
All lanes: SMAD1 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 53, 16 kDa
Observed band size: 53 kDa

IHC image of CSB-PA618998LA01HU diluted at 1:600 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with CSB-PA618998LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).