Western Blot
Positive WB detected in: K562 whole cell lysate(30μg), HEK293 whole cell lysate(30μg), A549 whole cell lysate(30μg), MCF-7 whole cell lysate(30μg), THP-1 whole cell lysate(30μg), HepG2 whole cell lysate(30μg), A431 whole cell lysate(30μg), HeLa whole cell lysate(30μg), Raji whole cell lysate(30μg)
All lanes: XBP1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 29 kDa
Observed band size: 35 kDa
Exposure time：2min

Overlay Peak curve showing MCF-7 cells stained with CSB-RA119872A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA05319A0Rb at dilution of 1:100

Immunohistochemistry of paraffin-embedded human pancreatic cancer using CSB-PA05319A0Rb at dilution of 1:100

Immunofluorescent analysis of Hela cells using CSB-PA05319A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)