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  4. Human N-terminal pro-brain natriuretic peptide,NT-proBNP ELISA KIT

Human N-terminal pro-brain natriuretic peptide,NT-proBNP ELISA KIT

  • 中文名稱：
    人N端前腦鈉素(NT-proBNP)酶聯免疫試劑盒
  • 貨號：
    CSB-E05152h
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    0.313 ng/ml-20 ng/ml
  • 靈敏度：
    0.216 ng/mL
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    人N端前腦鈉素(NT-proBNP)酶聯免疫試劑盒（CSB-E05152h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的NT-proBNP含量。NT-proBNP是一種重要的生物標志物。其背景在于它是腦鈉肽原經蛋白酶切割后的無活性片段，水平變化與心室壁壓力和容量負荷密切相關。研究機制上，常通過檢測血液中它的含量，輔助診斷心力衰竭、評估預后及指導治療。試劑盒檢測范圍為0.313 ng/ml-20 ng/ml，靈敏度為0.216 ng/mL。適用于心血管疾病機制研究、動物模型評估或體外實驗中NT - proBNP表達水平的動態監測本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    NT-proBNP
  • 種屬：
    Homo sapiens (Human)
  • 反應時間：
    1-5h
  • 上樣量：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

     

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays):CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

     

  • 線性度：

     

    To assess the linearity of the assay, samples were spiked with high concentrations of human NT-proBNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

  • 標準曲線：

     

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

     

  • 本試劑盒所含材料：
      • An assay plate --- The 96-well plate is composed of 12 x 8 strip plates and can be taken apart.
      • Two bottles of lyophilized standard --- Reconstitute one bottle of the standard with sample diluent at dilution series and draw the standard curve.
      • Biotin-labeled NT-proBNP antibody (100 x concentrate) 1 x 120 μl --- Act as the detection antibody and need to be diluted before use.
      • HRP-avidin (100 x concentrate) 1 x 120 μl --- Catalyze the TMB substrate for color development and need to be diluted before use.
      • Biotin-antibody Diluent 1 x 15 ml --- Dilute the Biotin-antibody.
      • HRP-avidin Diluent 1 x 15 ml --- Dilute the HRP-avidin.
      • Sample Diluent 1 x 50 ml --- Reconstitute the standard and dilute the sample to an appropriate concentration.
      • Wash Buffer (25 x concentrate) 1 x 20 ml --- Wash away the unbound solution and non-specific substances.
      • TMB Substrate 1 x 10 ml --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • Stop Solution 1 x 10 ml --- Add stop solution and stop the color development. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips(For 96 wells) --- Seal the microtiter plate when incubation.
      • An instruction manual

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  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 最新研究進展：
    NT-proBNP，全名為N末端B型利鈉肽前體，是一種由心臟細胞合成的激素分子。最近的研究表明，NT-proBNP可以作為心衰的標志物，可用于心衰的診斷、預測和治療監測。此外，NT-proBNP還參與了心血管疾病、腎臟疾病和糖尿病等多種疾病的發生和發展。